Keratin 17 knockdown suppressed malignancy and cisplatin tolerance of bladder cancer cells, as well as the activation of AKT and ERK pathway

Introduction. Bladder cancer (BCa) is one the most common urinary system malignancies and approximately quarter of diagnosis invasive muscle-invasive BCa. Accumulating evidence revealed that keratin 17 (KRT17) closely related to prognosis progression various tumors including a recent study also implying potential role KRT17 in However, specific BCa remains be elucidated. Material methods. The expression 5637 cells SV-HUC-1 normal human urothelial was detected using quantitative real-time PCR (qRT-PCR) western blot. Short hairpin RNA targeting used knockdown cells. colony formation assessed proliferation studied by Cell Counting Kit-8 (CCK-8). Invasion epithelial-mesenchymal transition (EMT) capacity were transwell assay blot, respectively. Cisplatin sensitivity measured evaluating cell viability CCK-8 assay. downstream pathway explored Results. elevated comparison with at mRNA protein levels. vitro assays demonstrated interference affected proliferation, invasion cells, as well EMT. Furthermore, enhanced cisplatin Mechanically, ablation led inactivation both AKT ERK pathways. Conclusions. Our results elucidate vital development malignancy probably activation pathways suggest it may represent novel therapeutic target for